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Revisi terkini sejak 8 Agustus 2022 01.40

Blot Western adalah sebuah metode untuk mendeteksi protein pada sampel jaringan. Imunoblot menggunakan elektroforesis gel untuk memisahkan protein asli atau perubahan oleh jarak polipeptida atau oleh struktur 3-D protein. Protein tersebut dikirim ke membran, di mana mereka dideteksi menggunakan antibodi untuk menargetkan protein.

Referensi

Renart J, Reiser J, Stark GR. "Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure", Proc Natl Acad Sci U S A, 1979 Jul;76(7):3116-20. PMID: 91164 abstract Diarsipkan 2008-03-23 di Wayback Machine.
Towbin H, Staehelin T, Gordon J. "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications" Proc Natl Acad Sci U S A, 1979 Sep;76(9):4350-4. PMID: 388439 abstract Diarsipkan 2008-03-17 di Wayback Machine.
Citation's Classic: Burnette

Pranala luar

Artikel bertopik biologi ini adalah sebuah rintisan. Anda dapat membantu Wikipedia dengan mengembangkannya.

@@ Baris 1: / Baris 1: @@
-[[Berkas:Western Blot.png|thumb|Sebuah western blot.]]
+[[Berkas:Western Blot.png|jmpl|Sebuah western blot.]]
 '''Blot Western''' adalah sebuah metode untuk mendeteksi [[protein]] pada sampel jaringan. Imunoblot menggunakan [[elektroforesis gel]] untuk memisahkan protein asli atau perubahan oleh jarak polipeptida atau oleh struktur 3-D protein. Protein tersebut dikirim ke membran, di mana mereka dideteksi menggunakan [[antibodi]] untuk menargetkan protein.
-== Lihat pula ==
-* [[Blot Southern]]
-* [[Blot Northern]]
-* [[Blot Eastern]]
 == Referensi ==
-* Renart J, Reiser J, Stark GR. "Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure", ''Proc&nbsp;Natl&nbsp;Acad&nbsp;Sci&nbsp;U&nbsp;S&nbsp;A'', 1979 Jul;76(7):3116-20. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=91164 PMID:&nbsp;91164] [http://www.pnas.org/cgi/content/abstract/76/7/3116 abstract]
+* Renart J, Reiser J, Stark GR. "Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure", ''Proc&nbsp;Natl&nbsp;Acad&nbsp;Sci&nbsp;U&nbsp;S&nbsp;A'', 1979 Jul;76(7):3116-20. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=91164 PMID:&nbsp;91164] [http://www.pnas.org/cgi/content/abstract/76/7/3116 abstract] {{Webarchive|url=https://web.archive.org/web/20080323132129/http://www.pnas.org/cgi/content/abstract/76/7/3116 |date=2008-03-23 }}
-* Towbin H, Staehelin T, Gordon J. "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications" ''Proc&nbsp;Natl&nbsp;Acad&nbsp;Sci&nbsp;U&nbsp;S&nbsp;A'', 1979 Sep;76(9):4350-4. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=388439 PMID:&nbsp;388439] [http://www.pnas.org/cgi/content/abstract/76/9/4350 abstract]
+* Towbin H, Staehelin T, Gordon J. "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications" ''Proc&nbsp;Natl&nbsp;Acad&nbsp;Sci&nbsp;U&nbsp;S&nbsp;A'', 1979 Sep;76(9):4350-4. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=388439 PMID:&nbsp;388439] [http://www.pnas.org/cgi/content/abstract/76/9/4350 abstract] {{Webarchive|url=https://web.archive.org/web/20080317111721/http://www.pnas.org/cgi/content/abstract/76/9/4350 |date=2008-03-17 }}
 * [http://www.garfield.library.upenn.edu/classics1991/A1991GK52400001.pdf Citation's Classic: Burnette]
-=== Pranala luar ===
+== Pranala luar ==
-* [http://www.natureprotocols.com/2006/09/15/western_blot_analysis_of_subce.php Western blot analysis of sub-cellular fractionated samples using the Odyssey Infrared Imaging System (a protocol)]
+* [http://www.natureprotocols.com/2006/09/15/western_blot_analysis_of_subce.php Western blot analysis of sub-cellular fractionated samples using the Odyssey Infrared Imaging System (a protocol)] {{Webarchive|url=https://web.archive.org/web/20100420005952/http://www.natureprotocols.com/2006/09/15/western_blot_analysis_of_subce.php |date=2010-04-20 }}
-* [http://www.prosci-inc.com/Western-Blot-Protocol.html Western blot protocol including example buffers and reprobing procedure]
+* [http://www.prosci-inc.com/Western-Blot-Protocol.html Western blot protocol including example buffers and reprobing procedure] {{Webarchive|url=https://web.archive.org/web/20071114213552/http://www.prosci-inc.com/Western-Blot-Protocol.html |date=2007-11-14 }}
+* http://kiatsehat2010.blogspot.co.id/2010/12/test-untuk-aids-elisa-dan-western-blot.html
+* http://wanenoor.blogspot.co.id/2012/12/metode-penelitian-dengan-cara-blot.html#.WW7VU9SGPcs
+* http://blogku-251294.blogspot.co.id/2015/04/test-elisa-atau-western-blot_23.html
+* https://belajarbiokimia.wordpress.com/2014/02/12/western-blot/
-{{biologi-stub}}
-[[Kategori:Teknik laboratorium]]
-[[Kategori:Biologi molekular]]
+{{biologi-stub}}
-[[ar:لطخة غربية]]
-[[ca:Transferència Western]]
+[[Kategori:Metode laboratorium]]
-[[cs:Western blot]]
+[[Kategori:Biologi molekuler]]
-[[de:Western Blot]]
+[[Kategori:Imunokimia]]
-[[en:Western blot]]
+[[Kategori:Virologi diagnostik]]
-[[eo:Proteina trimoo]]
-[[es:Western blot]]
-[[fa:وسترن بلات]]
-[[fr:Western blot]]
-[[he:תספיג חלבון]]
-[[hu:Western blot]]
-[[it:Western blot]]
-[[ja:ウェスタンブロッティング]]
-[[ko:웨스턴 블랏]]
-[[nl:Western blot]]
-[[pl:Western blot]]
-[[pt:Western blot]]
-[[ru:Вестерн блоттинг]]
-[[sv:Western blot]]
-[[ta:வெசுட்டர்ன் படிவு]]
-[[tr:Western blot]]
-[[ur:لطخۂ مغرب]]
-[[zh:西方墨點法]]